Experimental Biotechnology

Por: Swayam . en: , ,

Week 1: Basics of Laboratory Research:Good Lab Practices, Concept of buffering and Preparation of Solutions and ReagentsWeek 2: Electrophoresis (Part 1):Basic Concept of Electrophoresis, performance of electrophoresis and its applicationsWeek 3:Electrophoresis:Horizontal Gel electrophoresis, discussion about scientific questions and related experiments to solve them utilizing electrophoresisWeek 4: Chromatography (Part I): Analytical techniques in purification of biomolecules; Column chromatography, HPLC.Week 5: Chromatography (Part II) : Analytical techniques in purification of biomolecules; Column chromatography, HPLC.Week 6: Chromatography (Part III) : Discussion about scientific questions and related experiments to solve them utilizing chromatographyWeek 7:Immunology Techniques: Immunological tools, Antibody Generation and Purification, ELISA, Radial Immuno-diffusion, Western blotting and Immuno-precipitation. Week 8: Cell Biology Techniques (Part 1): Basics of cell-culture, isolation of cells from tissue and fractionation.Week 9: Cell Biology Techniques (Part 2): Immuno-localization, cell sorting, and discussion about scientific questions and related experiments to solve them utilizing cell biology techniques. Week 10:Molecular Biology (Part 1):Primer designing, Polymerase chain reaction, Different variants of PCR and their applications.Week 11:Molecular Biology (Part 2) Blotting Techniques, DNA and protein sequencing techniques and their applicationsWeek 12:Summary and Conclusions

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