ANIMAL BIOTECHNOLOGY

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Weeks 1
Day 1: Module 1- Introduction to Animal Biotechnology- Concept and Scope of BiotechnologyDay 2: Module 2 - Cloning vectors-I - Cosmids, Phagemids, Lambda Bacteriophage, M13, BAC, YAC, MACDay 3: Module 3 - Cloning vectors-II- Expression vectorsDay 4: InteractionDiscussion on Module 1, 2 & 3Day 5: Assignments
Weeks 2
Day 1: Module 4- Restriction enzymes IDay 2: Module 5 - Restriction enzymes types and detailed study of Type IIDay 3: Module 6 - Gene cloningDay 4: InteractionDiscussion on Module 4, 5 & 6Day 5: Assignments
Weeks 3
Day 1: Module 7- Gene transfer techniques
Day 2: Module 8 - Construction of genomic and cDNA librariesDay 3: Module 9 - screening of DNA LibrariesDay 4: InteractionDiscussion on Module 7, 8 & 9Day 5: Assignments
Weeks 4
Day 1: Module 10- Blotting techniques I (Southern and Northern)
Day 2: Module 11 - Blotting techniques II- Western blottingDay 3: Module 12 - DNA sequencingDay 4: InteractionDiscussion on Module 10, 11 & 12Day 5: Assignments
Weeks 5
Day 1: Module 13- Polymerase Chain ReactionDay 2: Module 14 - DNA Finger PrintingDay 3: Module 15 - DNA Micro array principle and technique.Day 4: InteractionDiscussion on Module 13, 14 & 15Day 5: Assignments
Weeks 6
Day 1: Module 16- Genetically modified organisms-I (Nuclear Transplantation)Day 2: Module 17 - Genetically modified organisms-II (Retroviral Method)Day 3: Module 18 - Genetically modified organisms-III (DNA microinjection)Day 4: InteractionDiscussion on Module 16, 17 & 18Day 5: Assignments

Weeks 7
Day 1: Module 19- Transgenic AnimalsDay 2: Module 20 - Applications of transgenic animals I- Production of PharmaceuticalsDay 3: Module 21- Applications of transgenic animals: production of donor organsDay 4: InteractionDiscussion on Module 19, 20 & 21Day 5: Assignments

Weeks 8
Day 1: Module 22- Applications of transgenic animals: knock out mice
Day 2: Module 23 - Production of transgenic plants- Agrobacterium mediated transformation
Day 3: Module 24 - Applications of transgenic plants: Insect and herbicide resistant plantsDay 4: InteractionDiscussion on Module 22, 23 & 24Day 5: Assignments

Weeks 9
Day 1: Module 25- Animal cell cultureDay 2: Module 26 - Expressing cloned genes in mammalian cellsDay 3: Module 27 - Diagnosis of genetic diseases (Cystic fibrosis, Sickle cell anemia)Day 4: InteractionDiscussion on Module 25, 26 & 27Day 5: Assignments

Weeks 10Day 1: Module 28- Recombinant DNA in medicine I- Recombinant insulin & Human growth hormoneDay 2: Module 29 – Gene TherapyDay 3: Module 30 - Animal propagation-– Artificial inseminationDay 4: InteractionDiscussion on Module 28, 29 & 30Day 5: Assignments

Weeks 11
Day 1: Module 31- Conservation Biology I- In vitro fertilization and embryo transfer in humans
Day 2: Module 32 - Conservation Biology II- In vitro fertilization and embryo transfer in farm animalsDay 3: Module 33 - Animal diseases need help of Biotechnology- Foot-and-mouth diseaseDay 4: InteractionDiscussion on Module 31, 32 & 33Day 5: Assignments
Weeks 12Day 1: Module 34- Genomic DNA isolation from E. Coli-PracticalDay 2: Module 35 - Plasmid DNA isolation- PracticalDay 3: Module 36 - Restriction digestion- PracticalDay 4: InteractionDiscussion on Module 34, 35 & 36Day5: Term end assessment

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